One Good Punch

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Four tips for a great kicker

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  1. Punch or kick to the groin;
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  4. punch | meaning of punch in Longman Dictionary of Contemporary English | LDOCE.

Topic clusters: how pub gigs can turn into sold-out stadium shows. How we work: designing tech company logos.

2. You're an alien for just one day.

Advice How to write Writing copywriter Want to write well? Open with a punch, close with a kick. Five tips for a great lede Open with a quote.

This is great advice from Writing to Deadline by Donald Murray. See my earlier summary of this essential book. Establish a sense of person.

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Start by stating a problem. This is the great trick of humourists like P. All right, I didn't. I glimpsed him in a crowd. I've been diagnosed with cancer, of a very treatable kind.

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Come to think of it -- as a drinking, smoking, saturated-fat hound -- my chance of survival has been improved by cancer. Current drugs for diabetic macular edema and wet age-related macular degeneration focus on halting the growth of these abnormal vessels to preserve what vision is left.

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The current standard of treatment is monthly injections directly into the eye to suppress new blood vessel growth. These frequent visits can be a burden for patients due to the discomfort, a small risk for each injection and, for some patients, difficulty getting to the appointment because their vision is not good enough to drive. The study was published in the Feb. In healthy eyes, the cells that make up blood vessels are bound together by proteins residing on the surface of the cell that are directed into place by Tie 2, another protein.

In diabetic macular edema, the Tie2 proteins disperse across the cell and no longer can maintain the fluid-tight barrier between the inside of a blood vessel and the outside.

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Gaps form between the cells, allowing fluids to permeate into the surrounding tissue. To understand how the drug they developed could strengthen these connections, the researchers designed a series of experiments to explore how AXT affects the control of Tie2 and the Velcro-like proteins.

In their first experiment, the researchers used cells derived from human blood vessels grown in the lab that mimicked those seen in wet age-related macular degeneration.

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  • When they added the AXT drug to these cells, the researchers found that AXT initiated a series of changes to cellular proteins. Using a technique to measure protein changes, the researchers found that Tie2 proteins seemed to migrate across the cell. Groups of Tie2 proteins began to congregate where cells met their neighbors, and began rebuilding connections with other blood vessel cells.

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    The researchers note that when observed under a microscope, the cells went from jagged-looking around the edges to having smooth and continuous outer edges that could be better suited for one cell to fit snugly against another. So they grew the cells in a single layer and tested whether fluid could pass through by pouring a fluorescent liquid on top of the cells and checking to see if any of the glowing liquid ended up underneath.

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    This showed the researchers that the drug helped blood vessel cells create a watertight seal between them. The researchers next wanted to see if the same effect could be achieved in living blood vessels. They used a fluorescent dye to observe the blood vessels in the eyes of normal mice and mice genetically engineered to mimic human macular degeneration. In the healthy mice, the researchers observed glowing blood vessels with crisp edges and very little fluorescence outside of the vessel.

    However, in the mice with macular degeneration, glowing liquids passed through the blood vessels, blurring the barrier between blood vessels and the surrounding tissues. After four days, the researchers found that in mice treated with AXT, about half as much of the fluorescent dye leaked from their vessels as in animals that received saline injections containing no drug.

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